John Rohde - Profile and Journalist Details
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John Rohde
Verified
Freelance Writer and Contributor, SoonerSports.com, Freelance
Edmond
Beats
Tennis AthleticsFootball
crowdfunding, b2b, enterprise
Content
A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth sgRNA
By Brett A. Duguay, John Rohde, Trinity H Tooley, Eric Stanley Pringle| bioRxiv@ AbstractCoronaviruses have large, positive-sense single-stranded RNA genomes that challenge conventional strategies for mutagenesis. Here, we report the development of a new reverse genetics system for the endemic human coronavirus (HCoV) OC43 that utilizes transformation-associated recombination (TAR) to assemble complete viral genomes from dsDNA genome fragments via homologous recombination in Saccharomyces cerevisiae.
By John Rohde| CityNewsOKC Verified Oklahoma City – There's only one Oklahoma. There's also only one softball program to win four straight NCAA championships. The Sooners officially capped the most dominant four-year span in Division I history Thursday night with an 8-4 victory over rival Texas at the Women's College World Series. The clincher came in Game Two of the best-of-three championship series before a crazed sellout crowd of 12,324 at Devon Park. OU has now won eight national crowns all-time and six of the last eight.
A yeast-based reverse genetics system to generate HCoV-OC43 reporter viruses encoding an eighth sgRNA
By Brett A. Duguay, John Rohde, Trinity H Tooley, Eric Stanley Pringle| bioRxiv@ AbstractCoronaviruses have large, positive-sense single-stranded RNA genomes that challenge conventional strategies for mutagenesis. Here, we report the development of a new reverse genetics system for the endemic human coronavirus (HCoV) OC43 that utilizes transformation-associated recombination (TAR) to assemble complete viral genomes from dsDNA genome fragments via homologous recombination in Saccharomyces cerevisiae.
Company Info
Freelance
Freelancer.com is the largest freelancing and crowdsourcing marketplace in the world, connecting over 81 million employers and freelancers across more than 247 countries. Founded in 2009 and based in Sydney, Australia, the platform allows employers to post jobs while freelancers can bid on these projects. It is publicly traded on the Australian Securities Exchange under the ticker ASX:FLN. The platform supports a variety of work categories, including software development, writing, design, and marketing. Freelancer.com also offers contests for creative work, where employers can award prize money to winning entries. Users can choose from different membership plans, including free and paid subscriptions, which provide various benefits. The mobile app enhances project management and communication for users on the go. With a diverse customer base that includes individuals, small businesses, and large enterprises, Freelancer.com plays a significant role in the global online economy.
De Pere, Wisconsin, United States